By Timothy Richard Parsons
An creation to the quantitative research of seawater, describing intimately organic and chemical ideas, that are thought of to be among these almost always utilized by organic oceanographers. The handbook presents entire directions for the addition of reagents and calculation of effects in regards fabric for every process in order that the unique texts should be consulted if helpful. regularly, the strategies require at least earlier expert education and techniques desiring very pricey gear were kept away from
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Extra resources for A Manual of Chemical & Biological Methods for Seawater Analysis
Cool to room temperature and make the volume to 500 ml with a little extra water. 5. Reducing reagent Mix 100 ml of metol-sulfite solution with 60 ml of oxalic acid solution. Add slowly, with mixing, 60 ml of the 50% sulfuric acid solution and make the mixture up to 300 ml with distilled water. The solution should be prepared each time for immediate use. F. Experimental procedure 1. Samples should be at room temperature (18-25°C). A d d 10 ml of molybdate solution to a dry 50-ml graduated cylinder fitted with a glass stopper.
0 ml portion (in triplicate) of the water sample into 10-ml 50 Soluble Organic Material capacity screw-capped test tubes with teflon liners. 05 ml of potassium borohydride solution into each test tube. Allow the solution to stand for 4 hr to complete reduction at 18°C in the dark. 2. 7N HC1. Remove the cap of the test tube and leave for 10 min at room temperature to allow hydrogen gas to evolve. The p H of the solution should be optimal at ca. 5 for the next step (Note d). 3. 1 ml of periodic acid solution into the sample solution and let stand for 10 min at room temperature in the dark.
34 g of potassium antimonyl-tartrate (tartar emetic) in 250 ml of water, warming if necessary. Store in a glass or plastic bottle. The solution is stable for many months. 5. Mixed reagent Mix together 100 ml ammonium molybdate, 250 ml sulfuric acid, 100 ml ascorbic acid, and 50 ml of potassium antimonyl-tartrate solutions. Prepare this reagent when needed and discard any excess. D o not store; the quantity is suitable for about 50 samples. F. Experimental procedure 1. W a r m the samples to room temperature (15-30°C).